Cell Signaling Complexin-1/2 (D8a6e) Rabbit mAb

Name: Cell Signaling Complexin-1/2 (D8a6e) Rabbit mAb
SKU: CSIG-28070S
Price: 354.51 USD

List Price $354.51 Your Price $354.51

Cell Signaling Complexin-1/2 (D8a6e) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-28070S

MFG.PART: 28070S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H M R
Sensitivity	Endogenous
Molecular Weight (kDa)	14-16
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:50, Immunofluorescence (Immunocytochemistry): 1:800}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Complexin-1/2 (D8A6E) Rabbit mAb recognizes endogenous levels of total complexin-1 and complexin-2 protein.
Species Reactivity	Human, Mouse, Rat
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro125 of human complexin-2 protein.
Background	Complexins are small soluble proteins composed of a central α-helical-structured domain surrounded by amino- and carboxy-terminal unstructured domains (1). These cytosolic proteins bind to t-SNAREs with low affinity and to assembled SNARE complexes with high affinity (1,2). Two isoforms, complexin-1 and complexin-2, are expressed in neuronal cells (3) where they regulate evoked and spontaneous exocytosis (4,5). Altered complexin expression resulting from RNAi-mediated knockdown (6) or gene invalidation (7) leads to alteration in spontaneous fusion events and neurotransmitter release, which reflects functions at both inhibitory and stimulatory synapses.

Specifications