Cell Signaling Oct-4a (C30a3) Rabbit mAb

Name: Cell Signaling Oct-4a (C30a3) Rabbit mAb
SKU: CSIG-2840T
Price: 149.48 USD

List Price $149.48 Your Price $149.48

Cell Signaling Oct-4a (C30a3) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-2840T

MFG.PART: 2840T

UNSPSC: 12352203

Manufacturer: Cell Signaling

Size	20 µl
Reactivity	H M
Sensitivity	Endogenous
Molecular Weight (kDa)	45
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000, Immunofluorescence (Immunocytochemistry): 1:200 - 1:400, Flow Cytometry (Fixed/Permeabilized): 1:100 - 1:400}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Oct-4A (C30A3) Rabbit mAb detects endogenous levels of total Oct-4A protein. Based on high sequence homology, this antibody may also detect OCT4-PG1.
Species Reactivity	Human, Mouse
Source/Purification	Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the amino terminus of human Oct-4A. The epitope has been mapped to residues surrounding Gly52.
Background	Oct-4 (POU5F1) is a transcription factor highly expressed in undifferentiated embryonic stem cells and embryonic germ cells (1). A network of key factors that includes Oct-4, Nanog, and Sox2 is necessary for the maintenance of pluripotent potential, and downregulation of Oct-4 has been shown to trigger cell differentiation (2,3). Research studies have demonstrated that Oct-4 is a useful germ cell tumor marker (4). Oct-4 exists as two splice variants, Oct-4A and Oct-4B (5). Recent studies have suggested that the Oct-4A isoform has the ability to confer and sustain pluripotency, while Oct-4B may exist in some somatic, non-pluripotent cells (6,7).