Cell Signaling Gfp (D5.1) Rabbit mAb

Name: Cell Signaling Gfp (D5.1) Rabbit mAb
SKU: CSIG-2956T
Price: 160.59 USD

List Price $160.59 Your Price $160.59

Cell Signaling Gfp (D5.1) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-2956T

MFG.PART: 2956T

UNSPSC: 12352203

Manufacturer: Cell Signaling

Size	20 µl
Reactivity	All
Sensitivity	Transfected Only
Molecular Weight (kDa)	27
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000, Immunohistochemistry (Paraffin): 1:100 - 1:400}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	GFP (D5.1) Rabbit mAb detects GFP, YFP, and CFP-tagged proteins exogenously expressed in cells. This antibody does not detect RFP-tagged proteins. Please note that the GFP, YFP, and CFP tags add approximately 27 kDa to the molecular weight of the fusion protein.
Species Reactivity	All Species Expected
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of GFP.
Background	Epitope tags are useful for the labeling and detection of proteins using immunoblotting, immunoprecipitation, and immunostaining techniques. Because of their small size, they are unlikely to affect the tagged protein’s biochemical properties.The green-fluorescent protein (GFP) functions as a bioluminescence energy transfer acceptor in the jellyfish Aequorea that maximally absorbs light at 395 nm and has an emission spectrum that peaks at 509 nm. GFP has become a very useful tool as a fusion protein that reports gene expression, traces cell lineage and defines subcellular protein localization (1).