Cell Signaling Phospho-Mtor (Ser2481) Antibody

Name: Cell Signaling Phospho-Mtor (Ser2481) Antibody
SKU: CSIG-2974T
Price: 151.50 USD

List Price $151.50 Your Price $151.50

Cell Signaling Phospho-Mtor (Ser2481) Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-2974T

MFG.PART: 2974T

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	20 µl
Reactivity	H M R Mk
Sensitivity	Endogenous
Molecular Weight (kDa)	289
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Phospho-mTOR (Ser2481) Antibody detects endogenous levels of mTOR only when phosphorylated at serine 2481.
Species Reactivity	Human, Mouse, Rat, Monkey
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser2481 of human mTOR. Antibodies are purified by protein A and peptide affinity chromatography.
Background	The mammalian target of rapamycin (mTOR, FRAP, RAFT) is a Ser/Thr protein kinase (1-3) that functions as an ATP and amino acid sensor to balance nutrient availability and cell growth (4,5). When sufficient nutrients are available, mTOR responds to a phosphatidic acid-mediated signal to transmit a positive signal to p70 S6 kinase and participate in the inactivation of the eIF4E inhibitor, 4E-BP1 (6). These events result in the translation of specific mRNA subpopulations. mTOR is phosphorylated at Ser2448 via the PI3 kinase/Akt signaling pathway and autophosphorylated at Ser2481 (7,8). mTOR plays a key role in cell growth and homeostasis and may be abnormally regulated in tumors. For these reasons, mTOR is currently under investigation as a potential target for anti-cancer therapy (9).

Specifications