Cell Signaling Eea1 (C45b10) Rabbit mAb

Name: Cell Signaling Eea1 (C45b10) Rabbit mAb
SKU: CSIG-3288T
Price: 153.52 USD

List Price $153.52 Your Price $153.52

Cell Signaling Eea1 (C45b10) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-3288T

MFG.PART: 3288T

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	20 µl
Reactivity	H M R
Sensitivity	Endogenous
Molecular Weight (kDa)	170
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:100, Immunofluorescence (Frozen): 1:50 - 1:200, Immunofluorescence (Immunocytochemistry): 1:50 - 1:200}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.For a carrier free (BSA and azide free) version of this product see product #65014.
Specificity/Sensitivity	EEA1 (C45B10) Rabbit mAb detects endogenous levels of total EEA1 protein.
Species Reactivity	Human, Mouse, Rat
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser70 of human EEA1 protein.
Background	EEA1 is an early endosomal marker and a Rab5 effector protein essential for early endosomal membrane fusion and trafficking (1-2). The carboxy terminus of EEA1 contains a FYVE domain which binds to phosphatidylinositol-3-phosphate (PtdIns(3)P), targeting EEA1 to early endosomes (3). The stable association of EEA1 with the endosomal membrane is regulated by PI3 kinase, Rab5 and calcium/calmodulin (4-6). Once on the membrane, EEA1 interacts with Rab5, NSF and syntaxin 13 to promote early endosomal membrane docking and fusion (7).

Specifications