Cell Signaling Dicer Antibody

Name: Cell Signaling Dicer Antibody
SKU: CSIG-3363T
Price: 137.36 USD

List Price $137.36 Your Price $137.36

Cell Signaling Dicer Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-3363T

MFG.PART: 3363T

UNSPSC: 12352203

Manufacturer: Cell Signaling

Size	20 µl
Reactivity	H
Sensitivity	Endogenous
Molecular Weight (kDa)	220
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:50}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Dicer Antibody detects endogenous levels of total Dicer protein.
Species Reactivity	Human
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human Dicer. Antibodies are purified by protein A and peptide affinity chromatography.
Background	Dicer is a member of the RNase III family that specifically cleaves double-stranded RNAs to generate microRNAs (miRNAs) (1). After long primary transcript pri-miRNAs are processed to stem-looped pre-miRNAs by Drosha (2), pre-miRNAs are transported to the cytoplasm and further processed by Dicer to produce 22-nucleotide mature miRNAs (3). The mature miRNA then becomes a part of the RNA-Induced Silencing Complex (RISC) and can bind to the 3 UTR of the target mRNA (3).