Cell Signaling Rad50 Antibody

Name: Cell Signaling Rad50 Antibody
SKU: CSIG-3427T
Price: 132.31 USD

List Price $132.31 Your Price $132.31

Cell Signaling Rad50 Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-3427T

MFG.PART: 3427T

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	20 µl
Reactivity	H Mk
Sensitivity	Endogenous
Molecular Weight (kDa)	153
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/ml BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Rad50 antibody detects endogenous levels of total Rad50 protein.
Species Reactivity	Human, Monkey
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Lys713 of human Rad50. Antibodies are purified by protein A and peptide affinity chromatography.
Background	The DNA repair protein Rad50 is a member of the structural maintenance of chromosomes family (SMC) and plays an important role in cell cycle checkpoint signaling and double-strand break repair in response to DNA damage (1-4). Rad50 forms a complex with Mre11 and Nbs1 that becomes activated in response to DNA damage (3). In normal human cells, the MRN complex acts to tether linear DNA molecules, providing a flexible link between DNA ends (1). Genomic instability and cancer have been shown to develop in cells with genetic mutations affecting the proteins in the MRN complex (2). ATM-dependent phosphorylation of Rad50 at Ser635 in response to DNA damage is important in regulating downstream signaling, DNA repair, and checkpoint control (5).

Specifications