Cell Signaling Ldha (C4b5) Rabbit mAb

Name: Cell Signaling Ldha (C4b5) Rabbit mAb
SKU: CSIG-3582T
Price: 153.52 USD

List Price $153.52 Your Price $153.52

Cell Signaling Ldha (C4b5) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-3582T

MFG.PART: 3582T

UNSPSC: 12352203

Manufacturer: Cell Signaling

Size	20 µl
Reactivity	H Mk
Sensitivity	Endogenous
Molecular Weight (kDa)	37
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000, Immunohistochemistry (Paraffin): 1:200 - 1:800, Immunofluorescence (Immunocytochemistry): 1:100 - 1:400}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.For a carrier free (BSA and azide free) version of this product see product #43723.
Specificity/Sensitivity	LDHA (C4B5) Rabbit mAb detects endogenous levels of total LDHA protein.
Species Reactivity	Human, Monkey
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human LDHA.
Background	Lactate dehydrogenase (LDH) catalyzes the interconversion of pyruvate and NADH to lactate and NAD+. When the oxygen supply is too low for mitochondrial ATP production, this reaction recycles NADH generated in glycolysis to NAD+, which reenters glycolysis. The major form of LDH found in muscle cells is the A (LDHA) isozyme. The LDHA promoter contains HIF-1α binding sites (1). LDHA expression is induced under hypoxic conditions (2). During intensive and prolonged muscle exercise, lactate accumulates in muscle cells when the supply of oxygen does not meet demand. When oxygen levels return to normal, LDH converts lactate to pyruvate to generate ATP in the mitochondrial electron transport chain.