Cell Signaling Phospho-Eef2k (Ser366) Antibody

Name: Cell Signaling Phospho-Eef2k (Ser366) Antibody
SKU: CSIG-3691T
Price: 149.48 USD

List Price $149.48 Your Price $149.48

Cell Signaling Phospho-Eef2k (Ser366) Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-3691T

MFG.PART: 3691T

UNSPSC: 12352203

Manufacturer: Cell Signaling

Size	20 µl
Reactivity	H R Mk
Sensitivity	Endogenous
Molecular Weight (kDa)	105
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:100}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Phospho-eEF2k (Ser366) Antibody detects endogenous levels of eEF2k only when phosphorylated at Ser366.
Species Reactivity	Human, Rat, Monkey
Source/Purification	Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser366 of human eEF2k. Antibodies are purified by protein A and peptide affinity chromatography.
Background	Eukaryotic elongation factor 2 kinase (eEF2k) phosphorylates and inactivates eEF2, resulting in the inhibition of peptide-chain elongation (1). eEF2k is normally dependent on Ca2+ ions and calmodulin (2,3). It can be activated by PKA in response to elevated cAMP levels (4-6), which are generally increased in stress- or starvation-related conditions. eEF2k can also be regulated in response to a wide range of stimuli that promote cell growth and protein synthesis. This involves the phosphorylation of eEF2k by p90RSK and p70 S6 kinase at Ser366 or by SAPK4/p38delta at Ser359, leading to the inactivation of eEF2k (7,8), which facilitates the dephosphorylation of eEF2, and thus promotes translation.