Cell Signaling Pdhk1 (C47h1) Rabbit mAb

Name: Cell Signaling Pdhk1 (C47h1) Rabbit mAb
SKU: CSIG-3820T
Price: 153.52 USD

List Price $153.52 Your Price $153.52

Cell Signaling Pdhk1 (C47h1) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-3820T

MFG.PART: 3820T

UNSPSC: 12352203

Manufacturer: Cell Signaling

Size	20 µl
Reactivity	H M R Mk
Sensitivity	Endogenous
Molecular Weight (kDa)	47
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:50}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	PDHK1 (C47H1) Rabbit mAb detects endogenous levels of total PDHK1 protein.
Species Reactivity	Human, Mouse, Rat, Monkey
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human PDHK1 protein.
Background	Pyruvate generated from glycolysis is converted to acetyl-CoA by pyruvate dehydrogenase (PDH) under normoxia (1-3). This is a critical link between glycolysis and the TCA cycle (3). PDH activity is regulated by phosphorylation and dephosphorylation (3). Pyruvate dehydrogenase kinase (PDHK) phosphorylates PDH and inactivates it, whereas dephosphorylation of PDH is carried out by pyruvate dehydrogenase phosphatase to generate the active form (3). Hypoxia can directly induce pyruvate dehydrogenase kinase 1 (PDHK1) expression, which results in inactivation of PDH and the TCA cycle and subsequent suppression of metabolism (4).