Cell Signaling Phospho-Sek1/Mkk4 (Ser257) (C36c11) Rabbit mAb

Name: Cell Signaling Phospho-Sek1/Mkk4 (Ser257) (C36c11) Rabbit mAb
SKU: CSIG-4514T
Price: 170.69 USD

List Price $170.69 Your Price $170.69

Cell Signaling Phospho-Sek1/Mkk4 (Ser257) (C36c11) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-4514T

MFG.PART: 4514T

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	20 µl
Reactivity	H M R Mk
Sensitivity	Endogenous
Molecular Weight (kDa)	44
Source/Isotype	Rabbit
Application/Dilution	{Western Blotting: 1:1000, Flow Cytometry (Fixed/Permeabilized): 1:400}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Phospho-SEK1/MKK4 (Ser257) (C36C11) Rabbit mAb detects endogenous levels of SEK1/MKK4 phosphorylated at Ser257 .
Species Reactivity	Human, Mouse, Rat, Monkey
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser257/Thr261 of human SEK1/MKK4.
Background	SAPK/Erk kinase (SEK1), also known as MKK4 or Jun kinase kinase (JNKK), activates the MAP kinase homologues SAPK and JNK in response to various cellular stresses and inflammatory cytokines (1-3). Activation of SEK1 occurs through MEKK phosphorylation of serine and threonine residues at positions 257 and 261, respectively. Like MEK, SEK is a dual-specificity protein kinase that phosphorylates SAPK/JNK at a conserved TPY site in its activation loop (4). Phosphorylation by Akt at Ser80 inhibits SEK1 and suppresses stress-activated signal transduction (5).

Specifications