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Cell Signaling Phospho-Nf-Kappab2 P100 (Ser866/870) Antibody
List Price
$149.48
Your Price
$149.48
Cell Signaling Phospho-Nf-Kappab2 P100 (Ser866/870) Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)
NETA PART:
CSIG-4810T
MFG.PART:
4810T
UNSPSC:
12352203
Manufacturer:
Cell Signaling
| Size | 20 µl |
| Reactivity | H M |
| Sensitivity | Transfected Only |
| Molecular Weight (kDa) | 110 |
| Source/Isotype | Rabbit |
| Application/Dilution | {Western Blotting: 1:1000, Immunoprecipitation: 1:50} |
| Storage | Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody. |
| Specificity/Sensitivity | Phospho-NF-κB2 p100 (Ser866/870) Antibody detects transfected NF-κB2 p100 when phosphorylated at serines 866 and 870. |
| Species Reactivity | Human, Mouse |
| Source/Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding serines 866/870 of NF-κB2 p100. Nomenclature refers to the human sequence (SwissProt# Q00653). This site is homologous to rat Ser864/868 (Q5U2Z4) and mouse Ser865/869 (Q9WTK5). Antibodies are purified by protein A and peptide affinity chromatography. |
| Background | Transcription factors of the nuclear factor κB (NF-κB)/Rel family play a pivotal role in inflammatory and immune responses (1,2). There are five family members in mammals: RelA, c-Rel, RelB, NF-κB1 (p105/p50), and NF-κB2 (p100/p52). Both p105 and p100 are proteolytically processed by the proteasome to produce p50 and p52, respectively. Rel proteins bind p50 and p52 to form dimeric complexes that bind DNA and regulate transcription. In unstimulated cells, NF-κB is sequestered in the cytoplasm by IκB inhibitory proteins (3-5). NF-κB-activating agents can induce the phosphorylation of IκB proteins, targeting them for rapid degradation through the ubiquitin-proteasome pathway and releasing NF-κB to enter the nucleus where it regulates gene expression (6-8). NIK and IKKα (IKK1) regulate the phosphorylation and processing of NF-κB2 (p100) to produce p52, which translocates to the nucleus (9-11). |
| SKU | CSIG-4810T |
|---|---|
| Supplier Part Number | 4810T |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSIG |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |