Cell Signaling Non-Phospho-4e-Bp1 (Thr46) (87d12) Rabbit mAb

Name: Cell Signaling Non-Phospho-4e-Bp1 (Thr46) (87d12) Rabbit mAb
SKU: CSIG-4923T
Price: 168.67 USD

List Price $168.67 Your Price $168.67

Cell Signaling Non-Phospho-4e-Bp1 (Thr46) (87d12) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-4923T

MFG.PART: 4923T

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	20 µl
Reactivity	H M R Mk
Sensitivity	Endogenous
Molecular Weight (kDa)	15-20
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000, Immunofluorescence (Immunocytochemistry): 1:200, Flow Cytometry (Fixed/Permeabilized): 1:200}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Non-phospho-4E-BP1 (Thr46) (87D12) Rabbit mAb detects endogenous levels of 4E-BP1 only when dephosphorylated at Thr46. The antibody cross-reacts with 4E-BP2 and 4E-BP3 dephosphorylated at equivalent sites.
Species Reactivity	Human, Mouse, Rat, Monkey
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Thr46 of human 4E-BP1.
Background	Translation repressor protein 4E-BP1 (also known as PHAS-1) inhibits cap-dependent translation by binding to the translation initiation factor eIF4E. Hyperphosphorylation of 4E-BP1 disrupts this interaction and results in activation of cap-dependent translation (1). Both the PI3 kinase/Akt pathway and FRAP/mTOR kinase regulate 4E-BP1 activity (2,3). Multiple 4E-BP1 residues are phosphorylated in vivo (4). While phosphorylation by FRAP/mTOR at Thr37 and Thr46 does not prevent the binding of 4E-BP1 to eIF4E, it is thought to prime 4E-BP1 for subsequent phosphorylation at Ser65 and Thr70 (5).

Specifications