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Cell Signaling Phospho-Cd79a (Tyr182) Antibody
List Price
$149.48
Your Price
$149.48
Cell Signaling Phospho-Cd79a (Tyr182) Antibody - CSIG (Additional S&H or Hazmat Fees May Apply)
NETA PART:
CSIG-5173T
MFG.PART:
5173T
UNSPSC:
12352203
Manufacturer:
Cell Signaling
| Size | 20 µl |
| Reactivity | H |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 45-55 |
| Source/Isotype | Rabbit |
| Application/Dilution | {Western Blotting: 1:1000, Immunoprecipitation: 1:50, Immunofluorescence (Immunocytochemistry): 1:200, Flow Cytometry (Fixed/Permeabilized): 1:100} |
| Storage | Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA and 50% glycerol. Store at –20°C. Do not aliquot the antibody. |
| Specificity/Sensitivity | Phospho-CD79A (Tyr182) Antibody detects endogenous levels of CD79A protein only when phosphorylated on Tyr188 of CD79A. This corresponds to Tyr182 on mouse CD79A protein. |
| Species Reactivity | Human |
| Source/Purification | Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr188 of human CD79A protein. This sequence corresponds to Tyr182 of mouse CD79A protein. Antibodies are purified by protein A and peptide affinity chromatography. |
| Background | Antigen receptors found on the surface of B cells contain a heterodimeric signaling component composed of CD79A and CD79B, also known as Ig α and Ig β, respectively (1,2). Presence of this receptor complex is essential for B cell development and function (3). Together these two proteins and the associated B cell receptor (BCR) initiate intracellular signaling following antigen binding (4,5). An immunoreceptor tyrosine-based activation motif (ITAM) found in the CD79A intracellular region appears to be important for its function (6). Antigen binding precedes formation of the CD79A and CD79B heterodimer and subsequent activation of receptor associated kinases (7). Research has shown that CD79A is a marker for B-lineage lymphoblastic leukemia (8). Additionally, investigators have found that mutations in the CD79A (MB1) gene are associated with abnormally low levels of functional B cell receptors in some cases of chronic B cell lymphocytic leukemia (9).Tyr182 of mouse CD79A (corresponding to Tyr188 of human CD79A) is one of two key tyrosine residues in the immunoreceptor tyrosine-based activation motif (ITAM) of CD79A. Tyrosines in the ITAM motifs are phosphorylated by Src family kinases (e.g., Lyn, Blk), and play a critical role in modulating signal transduction following immune receptor activation (10). |
| SKU | CSIG-5173T |
|---|---|
| Supplier Part Number | 5173T |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSIG |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |