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Cell Signaling Ezh2 (D2c9) Xp<LT/>Sup≫®<LT/>/Sup≫ Rabbit mAb
List Price $162.61 Your Price $162.61
Cell Signaling Ezh2 (D2c9) XpSup≫®/Sup≫ Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)
NETA PART: CSIG-5246T
MFG.PART: 5246T
UNSPSC: 12352203
Manufacturer: Cell Signaling
| Size | 20 µl |
| Reactivity | H M R Mk |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 98 |
| Source/Isotype | Rabbit IgG |
| Application/Dilution | {Western Blotting: 1:1000, Simple Western™: 1:10 - 1:50, Immunoprecipitation: 1:300, IHC Leica Bond: 1:50 - 1:200, Immunohistochemistry (Paraffin): 1:50, Immunofluorescence (Frozen): 1:100 - 1:400, Immunofluorescence (Immunocytochemistry): 1:100 - 1:400, Flow Cytometry (Fixed/Permeabilized): 1:100 - 1:400, Chromatin IP: 1:100, Chromatin IP-seq: 1:100, CUT&RUN: 1:100} |
| Storage | Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.For a carrier free (BSA and azide free) version of this product see product #16098. |
| Specificity/Sensitivity | Ezh2 (D2C9) XP® Rabbit mAb detects endogenous levels of total Ezh2 protein. This antibody does not cross-react with Ezh1 protein. |
| Species Reactivity | Human, Mouse, Rat, Monkey |
| Source/Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Arg354 of human Ezh2 protein. |
| Background | The polycomb group (PcG) proteins are involved in maintaining the silenced state of several developmentally regulated genes and contribute to the maintenance of cell identity, cell cycle regulation, and oncogenesis (1,2). Enhancer of zeste homolog 2 (Ezh2), a member of this large protein family, contains four conserved regions including domain I, domain II, and a cysteine-rich amino acid stretch that precedes the carboxy-terminal SET domain (3). The SET domain has been linked with histone methyltransferase (HMTase) activity. Moreover, mammalian Ezh2 is a member of a histone deacetylase complex that functions in gene silencing, acting at the level of chromatin structure (4). Ezh2 complexes methylate histone H3 at Lys9 and 27 in vitro, which is thought to be involved in targeting transcriptional regulators to specific loci (5). Ezh2 is deregulated in various tumor types, and its role, both as a primary effector and as a mediator of tumorigenesis, has become a subject of increased interest (6). |
| SKU | CSIG-5246T |
|---|---|
| Supplier Part Number | 5246T |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSIG |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |