Cell Signaling Wdr59 (D4z7a) Rabbit mAb

Name: Cell Signaling Wdr59 (D4z7a) Rabbit mAb
SKU: CSIG-53385S
Price: 354.51 USD

List Price $354.51 Your Price $354.51

Cell Signaling Wdr59 (D4z7a) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-53385S

MFG.PART: 53385S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Size	100 µl
Reactivity	H Mk
Sensitivity	Endogenous
Molecular Weight (kDa)	110
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:50}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	WDR59 (D4Z7A) rabbit mAb recognizes endogenous levels of total WDR59 protein.
Species Reactivity	Human, Monkey
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding His356 of human WDR59 protein.
Background	The mTORC1 kinase complex plays a critical role in cell growth regulation (1, 2). mTORC1 activity is modulated by cellular environmental factors (e.g., energy levels, growth factors, amino acids) (3, 4), which are mediated through a protein complex known as GATOR (GAP Activity Towards Rags) (5). GATOR is comprised of two protein subcomplexes (GATOR1 and GATOR2), that function in opposing fashion to regulate mTORC1 activity (5). WDR59 was identified as a component of the GATOR2 subcomplex (also containing Mios, WDR24, Seh1L and Sec13), that functions to positively regulate mTORC1 activity (5). Conversely, the GATOR1 subcomplex (containing DEPDC5, Nprl2 and Nprl3), negatively regulates mTORC1 activity, through activation of RagA and RagB GTPases (5).