Cell Signaling Dicer (D38e7) Rabbit mAb

Name: Cell Signaling Dicer (D38e7) Rabbit mAb
SKU: CSIG-5362T
Price: 153.52 USD

List Price $153.52 Your Price $153.52

Cell Signaling Dicer (D38e7) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-5362T

MFG.PART: 5362T

UNSPSC: 12352203

Manufacturer: Cell Signaling

Size	20 µl
Reactivity	H
Sensitivity	Endogenous
Molecular Weight (kDa)	220
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:50}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Dicer (D38E7) Rabbit mAb detects endogenous levels of total Dicer protein.
Species Reactivity	Human
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ala1133 of human Dicer.
Background	Dicer is a member of the RNase III family that specifically cleaves double-stranded RNAs to generate microRNAs (miRNAs) (1). After long primary transcript pri-miRNAs are processed to stem-looped pre-miRNAs by Drosha (2), pre-miRNAs are transported to the cytoplasm and further processed by Dicer to produce 22-nucleotide mature miRNAs (3). The mature miRNA then becomes a part of the RNA-Induced Silencing Complex (RISC) and can bind to the 3 UTR of the target mRNA (3).