Cell Signaling Synaptophysin (D35e4) Rabbit mAb

Name: Cell Signaling Synaptophysin (D35e4) Rabbit mAb
SKU: CSIG-5461T
Price: 150.49 USD

List Price $150.49 Your Price $150.49

Cell Signaling Synaptophysin (D35e4) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-5461T

MFG.PART: 5461T

UNSPSC: 12352203

Manufacturer: Cell Signaling

Size	20 µl
Reactivity	H M R
Sensitivity	Endogenous
Molecular Weight (kDa)	38
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:25}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Synaptophysin (D35E4) Rabbit mAb detects endogenous levels of total synaptophysin protein.
Species Reactivity	Human, Mouse, Rat
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide surrounding Ala230 of human synaptophysin protein.
Background	Synaptophysin (SYP) is a neuronal synaptic vesicle glycoprotein that is expressed in neuroendocrine cells and neoplasms (1). Synaptophysin contains four transmembrane domains that form a hexameric channel or gap junction-like pore (2). Synaptophysin binds to the SNARE protein synaptobrevin/VAMP, which prevents the inclusion of synaptobrevin in the synaptic vesicle fusion complex and creates a pool of synaptobrevin for exocytosis when synapse activity increases (3). Synaptophysin is also responsible for targeting synaptobrevin 2/VAMP2 to synaptic vesicles, a critical component of the fusion complex (4).