Cell Signaling Chop (D46f1) Rabbit mAb

Name: Cell Signaling Chop (D46f1) Rabbit mAb
SKU: CSIG-5554T
Price: 150.49 USD

List Price $150.49 Your Price $150.49

Cell Signaling Chop (D46f1) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-5554T

MFG.PART: 5554T

UNSPSC: 12352203

Manufacturer: Cell Signaling

Size	20 µl
Reactivity	M
Sensitivity	Endogenous
Molecular Weight (kDa)	27
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:50}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	CHOP (D46F1) Rabbit mAb detects endogenous levels of total CHOP protein.
Species Reactivity	Mouse
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu159 of human CHOP protein.
Background	CHOP was identified as a C/EBP-homologous protein that inhibits C/EBP and LAP in a dominant-negative manner (1). CHOP expression is induced by certain cellular stresses including starvation and the induced CHOP suppresses cell cycle progression from G1 to S phase (2). Later it was shown that, during ER stress, the level of CHOP expression is elevated and CHOP functions to mediate programmed cell death (3). Studies also found that CHOP mediates the activation of GADD34 and Ero1-Lα expression during ER stress. GADD34 in turn dephosphorylates phospho-Ser51 of eIF2α thereby stimulating protein synthesis. Ero1-Lα promotes oxidative stress inside the endoplasmic reticulum (ER) (4). The role of CHOP in the programmed cell death of ER-stressed cells is correlated with its role promoting protein synthesis and oxidative stress inside the ER (4).