Cell Signaling Bcl-2 (124) Mouse mAb (Alexa Fluor® 488 Conjugate)

Name: Cell Signaling Bcl-2 (124) Mouse mAb (Alexa Fluor® 488 Conjugate)
SKU: CSIG-59422S
Price: 469.65 USD

List Price $469.65 Your Price $469.65

Cell Signaling Bcl-2 (124) Mouse mAb (Alexa Fluor® 488 Conjugate) - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-59422S

MFG.PART: 59422S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl (50 tests)
Reactivity	H
Sensitivity	Endogenous
Source/Isotype	Mouse IgG1
Application/Dilution	{Flow Cytometry (Fixed/Permeabilized): 1:50}
Storage	Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze.
Specificity/Sensitivity	Bcl-2 (124) Mouse mAb (Alexa Fluor® 488 Conjugate) recognizes endogenous levels of total Bcl-2 protein.
Species Reactivity	Human
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Gly47 of human Bcl-2 protein.
Background	Bcl-2 exerts a survival function in response to a wide range of apoptotic stimuli through inhibition of mitochondrial cytochrome c release (1). It has been implicated in modulating mitochondrial calcium homeostasis and proton flux (2). Several phosphorylation sites have been identified within Bcl-2, including Thr56, Ser70, Thr74, and Ser87 (3). It has been suggested that these phosphorylation sites may be targets of the ASK1/MKK7/JNK1 pathway and that phosphorylation of Bcl-2 may be a marker for mitotic events (4,5). Mutation of Bcl-2 at Thr56 or Ser87 inhibits its anti-apoptotic activity during glucocorticoid-induced apoptosis of T lymphocytes (6). Interleukin-3 and JNK-induced Bcl-2 phosphorylation at Ser70 may be required for its enhanced anti-apoptotic functions (7).

Specifications