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Cell Signaling Ifn-Gamma (Xmg1.2) Rat mAb (PE Conjugate)
List Price $141.40 Your Price $141.40
Cell Signaling Ifn-Gamma (Xmg1.2) Rat mAb (PE Conjugate) - CSIG (Additional S&H or Hazmat Fees May Apply)
NETA PART: CSIG-62675S
MFG.PART: 62675S
UNSPSC: 12352203
Manufacturer: Cell Signaling
| Size | 100 µg |
| Reactivity | M |
| Sensitivity | Endogenous |
| Source/Isotype | Rat IgG1 kappa |
| Application/Dilution | {Flow Cytometry (Fixed/Permeabilized): 1:160} |
| Storage | Supplied in 10 mm NaH2PO4, 150 mm NaCl, 0.09% NaN3, 0.1% gelatin, pH 7.2. This product is stable for 12 months when stored at 4ºC. Do not aliquot the antibody. Protect from light. Do not freeze. |
| Specificity/Sensitivity | IFN-γ (XMG1.2) Rat mAb (PE Conjugate) recognizes endogenous levels of total IFN-γ protein. This antibody detects an epitope within the intracellular domain. |
| Species Reactivity | Mouse |
| Source/Purification | This monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation. |
| Background | IFN-γ plays key roles in both the innate and adaptive immune response. IFN-γ activates the cytotoxic activity of innate immune cells, such as macrophages and NK cells (1,2). IFN-γ production by NK cells and antigen presenting cells (APCs) promotes cell-mediated adaptive immunity by inducing IFN-γ production by T lymphocytes, increasing class I and class II MHC expression, and enhancing peptide antigen presentation (1). Due to differences in the degree of glycosylation, there are 3 forms of IFN-γ, with approximate molecular weights of 25, 20, and 15.5 kDa by SDS-PAGE (5). The anti-viral activity of IFN-γ is due to its induction of PKR and other regulatory proteins. Binding of IFN-γ to the IFNGR1/IFNGR2 complex promotes dimerization of the receptor complexes to form the (IFNGR1/IFNGR2)2 -IFN-γ dimer. Binding induces a conformational change in receptor intracellular domains and signaling involves Jak1, Jak2, and Stat1 (3). The critical role of IFN-γ in amplification of immune surveillance and function is supported by increased susceptibility to pathogen infection by IFN-γ or IFNGR knockout mice and in humans with inactivating mutations in IFNGR1 or IFNGR2. IFN-γ also appears to have a role in atherosclerosis (4). |
| SKU | CSIG-62675S |
|---|---|
| Supplier Part Number | 62675S |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer | Cell Signaling |
| MSDS URL | Click here |
| Temperature | +4C |
| CountryOfOrigin | United States |
| ProductLine | CSIG |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |