Cell Signaling Chk2 (D9c6) Xp Rabbit mAb

Name: Cell Signaling Chk2 (D9c6) Xp Rabbit mAb
SKU: CSIG-6334T
Price: 162.61 USD

List Price $162.61 Your Price $162.61

Cell Signaling Chk2 (D9c6) Xp Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-6334T

MFG.PART: 6334T

UNSPSC: 12352203

Manufacturer: Cell Signaling

Size	20 µl
Reactivity	H
Sensitivity	Endogenous
Molecular Weight (kDa)	62
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:100}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Chk2 (D9C6) Rabbit mAb recognizes endogenous levels of total Chk2 protein.
Species Reactivity	Human
Source/Purification	Monoclonal antibody is produced by immunizing animals with a recombinant protein specific to human Chk2 protein. The epitope has been mapped to the amino terminus of human Chk2 protein.
Background	Chk2 is the mammalian orthologue of the budding yeast Rad53 and fission yeast Cds1 checkpoint kinases (1-3). The amino-terminal domain of Chk2 contains a series of seven serine or threonine residues (Ser19, Thr26, Ser28, Ser33, Ser35, Ser50, and Thr68) each followed by glutamine (SQ or TQ motif). These are known to be preferred sites for phosphorylation by ATM/ATR kinases (4,5). After DNA damage by ionizing radiation (IR), UV irradiation, or hydroxyurea treatment, Thr68 and other sites in this region become phosphorylated by ATM/ATR (5-7). The SQ/TQ cluster domain, therefore, seems to have a regulatory function. Phosphorylation at Thr68 is a prerequisite for the subsequent activation step, which is attributable to autophosphorylation of Chk2 at residues Thr383 and Thr387 in the activation loop of the kinase domain (8).