Cell Signaling Wee1 (D10d2) Rabbit mAb (PE Conjugate)

Name: Cell Signaling Wee1 (D10d2) Rabbit mAb (PE Conjugate)
SKU: CSIG-64728S
Price: 475.71 USD

List Price $475.71 Your Price $475.71

Cell Signaling Wee1 (D10d2) Rabbit mAb (PE Conjugate) - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-64728S

MFG.PART: 64728S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl (50 tests)
Reactivity	H Mk
Sensitivity	Endogenous
Source/Isotype	Rabbit IgG
Application/Dilution	{Flow Cytometry (Fixed/Permeabilized): 1:50}
Storage	Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibodies. Protect from light. Do not freeze.
Specificity/Sensitivity	Wee1 (D10D2) Rabbit mAb (PE Conjugate) recognizes endogenous levels of total Wee1 protein.
Species Reactivity	Human, Monkey
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the amino terminus of human Wee1 protein.
Background	Entry of all eukaryotic cells into mitosis is regulated by activation of cdc2 kinase. The critical regulatory step in activating cdc2 during progression into mitosis appears to be dephosphorylation of Tyr15 and Thr14 (1,2). Phosphorylation at Tyr15 and Thr14 and inhibition of cdc2 is carried out by Wee1 and Myt1 protein kinases, while Tyr15 dephosphorylation and activation of cdc2 is carried out by the cdc25 phosphatase (1,3,4). Hyperphosphorylation and inactivation of Myt1 in mitosis suggests that one or more kinases activated at the G2/M transition negatively regulates Myt1 activity. Kinases shown to phosphorylate Myt1 include cdc2, p90RSK, Akt, and Plk1 (5-7).

Specifications