Cell Signaling Phospho-Rad17 (Ser645) (D5h5) Rabbit mAb

Name: Cell Signaling Phospho-Rad17 (Ser645) (D5h5) Rabbit mAb
SKU: CSIG-6981S
Price: 419.15 USD

List Price $419.15 Your Price $419.15

Cell Signaling Phospho-Rad17 (Ser645) (D5h5) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-6981S

MFG.PART: 6981S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H
Sensitivity	Endogenous
Molecular Weight (kDa)	80
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:200}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Phospho-Rad17 (Ser645) (D5H5) Rabbit mAb recognizes endogenous levels of Rad17 protein only when phosphorylated at Ser645.
Species Reactivity	Human
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser645 of human Rad17 protein.
Background	The human checkpoint protein Rad17 and its fission and budding yeast orthologues (Schizosaccharomyces pombe Rad17 and Saccharomyces cerevisiae Rad24, respectively) are involved in the activation of checkpoint signals in response to DNA damage or disruption of DNA synthesis (1-4). Treatment of human cells with genotoxic agents induces ATM/ATR-dependent phosphorylation of Rad17 at Ser635 and Ser645. Rad17 phosphorylation is a critical early event during checkpoint signaling in DNA-damaged cells (5-7).

Specifications