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Cell Signaling Phospho-Stat3 (Ser727) (D4x3c) Rabbit mAb (Alexa Fluor 647 Conjugate)
List Price $505.00 Your Price $505.00
Cell Signaling Phospho-Stat3 (Ser727) (D4x3c) Rabbit mAb (Alexa Fluor 647 Conjugate) - CSIG (Additional S&H or Hazmat Fees May Apply)
NETA PART: CSIG-71958S
MFG.PART: 71958S
UNSPSC: 12352203
Manufacturer: Cell Signaling
| Size | 100 µl (50 tests) |
| Reactivity | H |
| Sensitivity | Endogenous |
| Source/Isotype | Rabbit IgG |
| Application/Dilution | {Flow Cytometry (Fixed/Permeabilized): 1:50} |
| Storage | Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibody. Protect from light. Do not freeze. |
| Specificity/Sensitivity | Phospho-Stat3 (Ser727) (D4X3C) Rabbit mAb (Alexa Fluor® 647 Conjugate) recognizes endogenous levels of Stat3 protein only when phosphorylated at Ser727. |
| Species Reactivity | Human |
| Source/Purification | Monoclonal antibody is produced by immunizing animals with a synthetic phospho-peptide corresponding to residues surrounding Ser727 of human Stat3 protein. |
| Background | The Stat3 transcription factor is an important signaling molecule for many cytokines and growth factor receptors (1) and is required for murine fetal development (2). Research studies have shown that Stat3 is constitutively activated in a number of human tumors (3,4) and possesses oncogenic potential (5) and anti-apoptotic activities (3). Stat3 is activated by phosphorylation at Tyr705, which induces dimerization, nuclear translocation, and DNA binding (6,7). Transcriptional activation seems to be regulated by phosphorylation at Ser727 through the MAPK or mTOR pathways (8,9). Stat3 isoform expression appears to reflect biological function as the relative expression levels of Stat3α (86 kDa) and Stat3β (79 kDa) depend on cell type, ligand exposure, or cell maturation stage (10). It is notable that Stat3β lacks the serine phosphorylation site within the carboxy-terminal transcriptional activation domain (8). |
| SKU | CSIG-71958S |
|---|---|
| Supplier Part Number | 71958S |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer | Cell Signaling |
| MSDS URL | Click here |
| Temperature | +4C |
| CountryOfOrigin | United States |
| ProductLine | CSIG |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 8.000000 |
| Lead Time | 5 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |