Cell Signaling Cleaved Caspase-3 (Asp175) (D3e9) Rabbit mAb (BSA And Azide Free)

Name: Cell Signaling Cleaved Caspase-3 (Asp175) (D3e9) Rabbit mAb (BSA And Azide Free)
SKU: CSIG-74860SF
Price: 906.98 USD

List Price $906.98 Your Price $906.98

Cell Signaling Cleaved Caspase-3 (Asp175) (D3e9) Rabbit mAb (BSA And Azide Free) - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-74860SF

MFG.PART: 74860SF

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µg
Reactivity	H M
Sensitivity	Endogenous
Source/Isotype	Rabbit IgG
Application/Dilution	{}
Storage	Supplied in 1X PBS, BSA and Azide Free. For standard formulation of this product see product #9579.
Specificity/Sensitivity	Cleaved Caspase-3 (Asp175) (D3E9) Rabbit mAb (BSA and Azide Free) recognizes endogenous levels of caspase-3 protein only when cleaved at Asp175. This antibody is preferred for immunofluorescence, flow cytometry, and immunohistochemistry applications. This antibody detects non-specific caspase substrates by western blot. Non-specific labeling may be observed by immunofluorescence in specific sub-types of healthy cells in fixed-frozen tissues (e.g., pancreatic alpha-cells). Nuclear background may be observed in rat and monkey samples.
Species Reactivity	Human, Mouse
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp175 of human caspase-3 protein.
Background	Caspase-3 (CPP-32, Apopain, Yama, SCA-1) is a critical executioner of apoptosis, as it is either partially or totally responsible for the proteolytic cleavage of many key proteins, such as the nuclear enzyme poly (ADP-ribose) polymerase (PARP) (1). Activation of caspase-3 requires proteolytic processing of its inactive zymogen into activated p17 and p12 fragments. Cleavage of caspase-3 requires the aspartic acid residue at the P1 position (2).

Specifications