Cell Signaling Phospho-Pkd/Pkcmu (Ser910) (E7m8c) Rabbit mAb

Name: Cell Signaling Phospho-Pkd/Pkcmu (Ser910) (E7m8c) Rabbit mAb
SKU: CSIG-75148S
Price: 405.01 USD

List Price $405.01 Your Price $405.01

Cell Signaling Phospho-Pkd/Pkcmu (Ser910) (E7m8c) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-75148S

MFG.PART: 75148S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Description

Size	100 µl
Reactivity	H M
Sensitivity	Endogenous
Molecular Weight (kDa)	115
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000}
Storage	Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Phospho-PKD/PKCμ (Ser910) (E7M8C) Rabbit mAb detects endogenous levels of PKD/PKCμ protein only when phosphorylated at Ser910.
Species Reactivity	Human, Mouse
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Ser910 of human PKD/PKCμ protein. This site is homologous to Ser916 in mouse sequence.
Background	Activation of PKC is one of the earliest events in a cascade leading to a variety of cellular responses, such as secretion, gene expression, proliferation, and muscle contraction (1,2). Protein kinase D (PKD), also called PKCμ, is a serine/threonine kinase whose activation is dependent on the phosphorylation of two activation loop sites, Ser744 and Ser748, via a PKC-dependent signaling pathway (3-5). In addition to the two activation loop sites, the carboxy-terminal Ser916 has been identified as an autophosphorylation site for PKD/PKCμ. Phosphorylation at Ser916 correlates with PKD/PKCμ catalytic activity (6).

Specifications