Cell Signaling Irak1 (D51g7) Rabbit mAb (PE Conjugate)

Name: Cell Signaling Irak1 (D51g7) Rabbit mAb (PE Conjugate)
SKU: CSIG-77143S
Price: 475.71 USD

List Price $475.71 Your Price $475.71

Cell Signaling Irak1 (D51g7) Rabbit mAb (PE Conjugate) - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-77143S

MFG.PART: 77143S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Size	100 µl (50 tests)
Reactivity	H M Mk
Sensitivity	Endogenous
Source/Isotype	Rabbit IgG
Application/Dilution	{Flow Cytometry (Fixed/Permeabilized): 1:50}
Storage	Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibodies. Protect from light. Do not freeze.
Specificity/Sensitivity	IRAK1 (D51G7) Rabbit mAb (PE Conjugate) detects endogenous levels of total IRAK1 protein.
Species Reactivity	Human, Mouse, Monkey
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxyl terminus of mouse IRAK1.
Background	Interleukin-1 (IL-1) receptor-associated kinase (IRAK) is a serine/threonine-specific kinase that can be coprecipitated in an IL-1-inducible manner with the IL-1 receptor (1). The mammalian family of IRAK molecules contains four members (IRAK1, IRAK2, IRAK3/IRAK-M, and IRAK4). The binding of IL-1 to IL-1 receptor type I (IL-1RI) initiates the formation of a complex that includes IL-1RI, AcP, MyD88, and IRAKs (2). IRAK undergoes autophosphorylation shortly after IL-1 stimulation. The subsequent events involve IRAK dissociation from the IL-1RI complex, its ubiquitination, and its association with two membrane-bound proteins: TAB2 and TRAF6. The resulting IRAK-TRAF6-TAB2 complex is then released into the cytoplasm where it activates protein kinase cascades, including TAK1, IKKs, and the stress-activated kinases (3).