Cell Signaling Cleaved-Il-1beta (Asp116) (D3a3z) Rabbit mAb

Name: Cell Signaling Cleaved-Il-1beta (Asp116) (D3a3z) Rabbit mAb
SKU: CSIG-83186S
Price: 416.12 USD

List Price $416.12 Your Price $416.12

Cell Signaling Cleaved-Il-1beta (Asp116) (D3a3z) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-83186S

MFG.PART: 83186S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Size	100 µl
Reactivity	H
Sensitivity	Endogenous
Molecular Weight (kDa)	17
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000, Immunofluorescence (Immunocytochemistry): 1:800 - 1:3200}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Cleaved-IL-1β (Asp116) (D3A3Z) Rabbit mAb recognizes endogenous levels of mature IL-1β protein only when cleaved at Asp116.
Species Reactivity	Human
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp116 of human IL-1β protein.
Background	Interleukin-1β (IL-1β), one of the major caspase-1 targets, is a multifunctional cytokine that is involved in a host of immune and proinflammatory responses (1). It is produced primarily by activated monocytes and macrophages. It signals through various adaptor proteins and kinases that lead to activation of numerous downstream targets (2-6). Human IL-1β is synthesized as a 31 kDa precursor. To gain activity, the precursor must be cleaved by caspase-1 between Asp116 and Ala117 to yield a 17 kDa mature form (7,8). Detection of the 17 kDa mature form of IL-1β is a good indicator of caspase-1 activity.