Cell Signaling Oct-4a (D6c8t) Rabbit mAb (Mouse Specific)

Name: Cell Signaling Oct-4a (D6c8t) Rabbit mAb (Mouse Specific)
SKU: CSIG-83932T
Price: 153.52 USD

List Price $153.52 Your Price $153.52

Cell Signaling Oct-4a (D6c8t) Rabbit mAb (Mouse Specific) - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-83932T

MFG.PART: 83932T

UNSPSC: 12352203

Manufacturer: Cell Signaling

Size	20 µl
Reactivity	M
Sensitivity	Endogenous
Molecular Weight (kDa)	45
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:100, Immunofluorescence (Immunocytochemistry): 1:800, Flow Cytometry (Fixed/Permeabilized): 1:200, Chromatin IP: 1:50}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Oct-4A (D6C8T) Rabbit mAb recognizes endogenous levels of total murine Oct-4A protein.
Species Reactivity	Mouse
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val80 of murine Oct-4A protein.
Background	Oct-4 (POU5F1) is a transcription factor highly expressed in undifferentiated embryonic stem cells and embryonic germ cells (1). A network of key factors that includes Oct-4, Nanog, and Sox2 is necessary for the maintenance of pluripotent potential, and downregulation of Oct-4 has been shown to trigger cell differentiation (2,3). Research studies have demonstrated that Oct-4 is a useful germ cell tumor marker (4). Oct-4 exists as two splice variants, Oct-4A and Oct-4B (5). Recent studies have suggested that the Oct-4A isoform has the ability to confer and sustain pluripotency, while Oct-4B may exist in some somatic, non-pluripotent cells (6,7).