Cell Signaling Cleaved Caspase-7 (Asp198) (D6h1) Rabbit mAb

Name: Cell Signaling Cleaved Caspase-7 (Asp198) (D6h1) Rabbit mAb
SKU: CSIG-8438T
Price: 170.69 USD

List Price $170.69 Your Price $170.69

Cell Signaling Cleaved Caspase-7 (Asp198) (D6h1) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-8438T

MFG.PART: 8438T

UNSPSC: 12352203

Manufacturer: Cell Signaling

Size	20 µl
Reactivity	H M R
Sensitivity	Endogenous
Molecular Weight (kDa)	18
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000, Immunofluorescence (Immunocytochemistry): 1:1600, Flow Cytometry (Fixed/Permeabilized): 1:1600}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Cleaved Caspase-7 (Asp198) (D6H1) Rabbit mAb recognizes endogenous levels of caspase-7 protein only when cleaved at Asp198.
Species Reactivity	Human, Mouse, Rat
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp198 of human caspase-7 protein.
Background	Caspase-7 (CMH-1, Mch3, ICE-LAP3) has been identified as a major contributor to the execution of apoptosis (1-4). Caspase-7, like caspase-3, is an effector caspase that is responsible for cleaving downstream substrates such as (ADP-ribose) polymerase and PARP (1,3). During apoptosis, caspase-7 is activated through proteolytic processing by upstream caspases at Asp23, Asp198, and Asp206 to produce the mature subunits (1,3). Similar to caspase-2 and -3, caspase-7 preferentially cleaves substrates following the recognition sequence DEVD (5).