Cell Signaling Phospho-Gsk-3beta (Ser9) (D85e12) Xp Rabbit mAb (PE Conjugate)

Name: Cell Signaling Phospho-Gsk-3beta (Ser9) (D85e12) Xp Rabbit mAb (PE Conjugate)
SKU: CSIG-8466S
Price: 497.93 USD

List Price $497.93 Your Price $497.93

Cell Signaling Phospho-Gsk-3beta (Ser9) (D85e12) Xp Rabbit mAb (PE Conjugate) - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-8466S

MFG.PART: 8466S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Size	100 µl (50 tests)
Reactivity	H M R Hm
Sensitivity	Endogenous
Source/Isotype	Rabbit IgG
Application/Dilution	{Flow Cytometry (Fixed/Permeabilized): 1:50}
Storage	Supplied in PBS (pH 7.2), less than 0.1% sodium azide and 2 mg/mL BSA. Store at 4°C. Do not aliquot the antibodies. Protect from light. Do not freeze.
Specificity/Sensitivity	Phospho-GSK-3β (Ser9) (D85E12) XP® Rabbit mAb detects endogenous levels of GSK-3β only when phosphorylated at Ser9. This antibody reacts with denatured components of bovine serum, including BSA.
Species Reactivity	Human, Mouse, Rat, Hamster
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser9 of human GSK-3β protein.
Background	Glycogen synthase kinase-3 (GSK-3) was initially identified as an enzyme that regulates glycogen synthesis in response to insulin (1). GSK-3 is a ubiquitously expressed serine/threonine protein kinase that phosphorylates and inactivates glycogen synthase. GSK-3 is a critical downstream element of the PI3K/Akt cell survival pathway whose activity can be inhibited by Akt-mediated phosphorylation at Ser21 of GSK-3α and Ser9 of GSK-3β (2,3). GSK-3 has been implicated in the regulation of cell fate in Dictyostelium and is a component of the Wnt signaling pathway required for Drosophila, Xenopus, and mammalian development (4). GSK-3 has been shown to regulate cyclin D1 proteolysis and subcellular localization (5).