Cell Signaling Phospho-Fak (Tyr397) (D20b1) Rabbit mAb

Name: Cell Signaling Phospho-Fak (Tyr397) (D20b1) Rabbit mAb
SKU: CSIG-8556T
Price: 170.69 USD

List Price $170.69 Your Price $170.69

Cell Signaling Phospho-Fak (Tyr397) (D20b1) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-8556T

MFG.PART: 8556T

UNSPSC: 12352203

Manufacturer: Cell Signaling

Size	20 µl
Reactivity	H
Sensitivity	Endogenous
Molecular Weight (kDa)	125
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:50}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Phospho-FAK (Tyr397) (D20B1) Rabbit mAb detects FAK protein only when phosphorylated at Tyr397. This antibody may cross-react with overexpressed tyrosine phosphorylated proteins such as EGFR.
Species Reactivity	Human
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr397 of human FAK protein.
Background	Focal adhesion kinase (FAK) is a widely expressed cytoplasmic protein tyrosine kinase involved in integrin-mediated signal transduction. It plays an important role in the control of several biological processes, including cell spreading, migration, and survival (1). Activation of FAK by integrin clustering leads to autophosphorylation at Tyr397, which is a binding site for the Src family kinases PI3K and PLCγ (2-5). Recruitment of Src family kinases results in the phosphorylation of Tyr407, Tyr576, and Tyr577 in the catalytic domain, and Tyr871 and Tyr925 in the carboxy-terminal region of FAK (6,7).