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Cell Signaling Col5a1 (E6u9w) Rabbit mAb
List Price $161.60 Your Price $161.60
Cell Signaling Col5a1 (E6u9w) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)
NETA PART: CSIG-86903T
MFG.PART: 86903T
UNSPSC: 12352203
Manufacturer: Cell Signaling
| Size | 20 µl |
| Reactivity | H |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 250 |
| Source/Isotype | Rabbit IgG |
| Application/Dilution | {Western Blotting: 1:1000, Immunoprecipitation: 1:100, IHC Leica Bond: 1:50 - 1:200, Immunohistochemistry (Paraffin): 1:50 - 1:200, Immunofluorescence (Immunocytochemistry): 1:200 - 1:800} |
| Storage | Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.For a carrier free (BSA and azide free) version of this product see product #80085. |
| Specificity/Sensitivity | COL5A1 (E6U9W) Rabbit mAb recognizes endogenous levels of total COL5A1 protein. Nonspecific staining was observed in skeletal muscle by immunohistochemistry. |
| Species Reactivity | Human |
| Source/Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Pro364 of human COL5A1 protein. |
| Background | The Extracellular Matrix (ECM) is a complex network of macromolecules that provides structural tissue support to cells in the basement membrane and interstitial matrix. It is composed of many molecules including proteins, glycoproteins, proteoglycans, and polysaccharides (1,2). One of the major proteins that comprises the ECM, and the human body, is collagen. Collagens are a large family of proteins. They are trimeric molecules comprised of three alpha polypeptide chains that form a triple helix structure that is characteristic of all collagens (3). The large family of collagens is divided into three subgroups: the fibrillar collagens, the non-fibril forming collagens, and the fibril-associated collagens. These sub groups differ in their structure and supramolecular assembly (3). COL5A1 is a minor fibrillar collagen expressed broadly across tissues. It participates in bone and cartilage development, and defects in the COL5A1 gene leads to Ehlers-Danlos Syndrome, a condition that affects the integrity of bones, cartilage, and skin (4,5). Several proteomics, gene profiling, and biochemical studies have indicated that aberrant COL5A1 may play a malignant role in various types of cancer including breast, renal, colon, prostate, lung, and others (6-10). |
| SKU | CSIG-86903T |
|---|---|
| Supplier Part Number | 86903T |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSIG |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |