Cell Signaling Mdm2 (D1v2z) Rabbit mAb

Name: Cell Signaling Mdm2 (D1v2z) Rabbit mAb
SKU: CSIG-86934S
Price: 354.51 USD

List Price $354.51 Your Price $354.51

Cell Signaling Mdm2 (D1v2z) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-86934S

MFG.PART: 86934S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Size	100 µl
Reactivity	H
Sensitivity	Endogenous
Molecular Weight (kDa)	90
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000, Immunoprecipitation: 1:100, Immunofluorescence (Immunocytochemistry): 1:200 - 1:800}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	MDM2 (D1V2Z) Rabbit mAb recognizes endogenous levels of total MDM2 protein. Based on the sequence of the peptide antigen, this antibody is expected to detect full-length MDM2, as well as isoforms A, C, alpha, F, g, and 11. This antibody may detect isoform A1.
Species Reactivity	Human
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val280 of human MDM2 protein.
Background	MDM2, a ubiquitin ligase for p53, plays a central role in regulation of the stability of p53 (1). Akt-mediated phosphorylation of MDM2 at Ser166 and Ser186 increases its interaction with p300, allowing MDM2-mediated ubiquitination and degradation of p53 (2-4). Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53 (3).