Cell Signaling Phospho-Marcks (Ser167/170) (D13e4) Xp Rabbit mAb

Name: Cell Signaling Phospho-Marcks (Ser167/170) (D13e4) Xp Rabbit mAb
SKU: CSIG-8722T
Price: 182.81 USD

List Price $182.81 Your Price $182.81

Cell Signaling Phospho-Marcks (Ser167/170) (D13e4) Xp Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-8722T

MFG.PART: 8722T

UNSPSC: 12352203

Manufacturer: Cell Signaling

Size	20 µl
Reactivity	H M R
Sensitivity	Endogenous
Molecular Weight (kDa)	75 rodent, 80 human
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000, Immunofluorescence (Immunocytochemistry): 1:100, Flow Cytometry (Fixed/Permeabilized): 1:200}
Storage	Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody.
Specificity/Sensitivity	Phospho-MARCKS (Ser167/170) (D13E4) XP® Rabbit mAb recognizes endogenous levels of MARCKS protein only when phosphorylated at Ser167 and Ser170. This antibody may also detect MARCKS mono-phosphorylated at Ser167.
Species Reactivity	Human, Mouse, Rat
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser167 and Ser170 of human MARCKS protein.
Background	Myristoylated Alanine-Rich C-Kinase Substrate (MARCKS) is a major PKC substrate expressed in many cell types. MARCKS has been implicated in cell motility, cell adhesion, phagocytosis, membrane traffic, and mitogenesis (1). PKC phosphorylates Ser159, 163, 167, and 170 of MARCKS in response to growth factors and oxidative stress. Phosphorylation at these sites regulates the calcium/calmodulin binding and filamentous (F)-actin cross-linking activities of MARCKS (2-4). Phosphorylation by PKC also results in translocation of MARCKS from the plasma membrane to the cytoplasm (5).