Cell Signaling Pras40 (D23c7) Xp Rabbit mAb (Biotinylated)

Name: Cell Signaling Pras40 (D23c7) Xp Rabbit mAb (Biotinylated)
SKU: CSIG-8858S
Price: 469.65 USD

List Price $469.65 Your Price $469.65

Cell Signaling Pras40 (D23c7) Xp Rabbit mAb (Biotinylated) - CSIG (Additional S&H or Hazmat Fees May Apply)

NETA PART: CSIG-8858S

MFG.PART: 8858S

UNSPSC: 12352203

Manufacturer: Cell Signaling

Size	100 µl
Reactivity	H M R Mk
Sensitivity	Endogenous
Molecular Weight (kDa)	40
Source/Isotype	Rabbit IgG
Application/Dilution	{Western Blotting: 1:1000}
Storage	Supplied in 136 mm NaCl, 2.6 mm KCI, 12 mm sodium phosphate (pH 7.4) dibasic, 2 mg/mL BSA, and 50% glycerol. Store at –20°C. Do not aliquot the antibodies.
Specificity/Sensitivity	PRAS40 (D23C7) XP® Rabbit mAb (Biotinylated) detects endogenous levels of total PRAS40 protein.
Species Reactivity	Human, Mouse, Rat, Monkey
Source/Purification	Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence of human PRAS40 protein.
Background	Many growth factors and hormones induce the phosphoinositide 3-kinase signaling pathway, which results in the activation of downstream effector proteins such as the serine/threonine kinase Akt (1,2). One known Akt substrate is a 40 kDa, proline-rich protein (PRAS40) that binds to 14-3-3 proteins (2). PRAS40 also binds mTOR to transduce Akt signals to the mTOR complex. Inhibition of mTOR signaling stimulates PRAS40 binding to mTOR, which in turn inhibits mTOR activity (3). PRAS40 interacts with raptor in mTOR complex 1 (mTORC1) in insulin-deprived cells and inhibits the activation of the mTORC1 pathway mediated by the cell cycle protein Rheb. Phosphorylation of PRAS40 by Akt at Thr246 relieves PRAS40 inhibition of mTORC1 (4). mTORC1 in turn phosphorylates PRAS40 at Ser183 (5).