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Cell Signaling Citrullinated Histone H3 (Arg17) (E4o3f) Rabbit mAb
List Price
$418.14
Your Price
$418.14
Cell Signaling Citrullinated Histone H3 (Arg17) (E4o3f) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)
NETA PART:
CSIG-97272S
MFG.PART:
97272S
UNSPSC:
12352203
Manufacturer:
Cell Signaling
| Size | 100 µl |
| Reactivity | H M |
| Sensitivity | Endogenous (IHC-P), Transfected (W) |
| Molecular Weight (kDa) | 17 |
| Source/Isotype | Rabbit IgG |
| Application/Dilution | {Western Blotting: 1:1000, Immunohistochemistry (Paraffin): 1:800 - 1:3200} |
| Storage | Supplied in 10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100 µg/mL BSA, 50% glycerol, and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. |
| Specificity/Sensitivity | Citrullinated Histone H3 (Arg17) (E4O3F) Rabbit mAb recognizes histone H3 citrullinated at residue Arg17. This antibody may also recognize histone H3 citrullinated at residue Arg26, but does not cross-react with any other known citrullinated or methylated arginine residues on histone H3. This antibody may detect a band of unknown identity at 38 kDa. |
| Species Reactivity | Human, Mouse |
| Source/Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of histone H3 in which Arg17 is citrullinated. |
| Background | The nucleosome, made up of four core histone proteins (H2A, H2B, H3, and H4), is the primary building block of chromatin. Histone posttranslational modifications, such as methylation, acetylation, ubiquitination, and citrullination, influence chromatin accessibility and regulate gene expression (1). Histone citrullination is carried out by protein arginine deiminases (PADs), which convert positively charged arginine residues into neutral citrulline (2). Histone citrullination affects the binding of histone reader proteins to chromatin, and it is associated with both transcriptional activation and repression (3-5). Histone citrullination is also a major marker for NETosis, which is a programmed cell death mechanism carried out by neutrophils (6). De-regulation of NETosis is associated with multiple autoimmune diseases, including rheumatoid arthritis (RA) and lupus (7,8). Histone hypercitrullination via PAD4 may also play a role in the progression of various cancers, including multiple myeloma (MM), acute myeloid leukemia (AML), lung cancer, and hepatocellular carcinoma (HCC) (9-12). |
| SKU | CSIG-97272S |
|---|---|
| Featured | No |
| Supplier Part Number | 97272S |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSIG |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| ACT Ecolabel | No |