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Cell Signaling Cd3epsilon (D4v8l) Rabbit mAb
List Price $167.66 Your Price $167.66
Cell Signaling Cd3epsilon (D4v8l) Rabbit mAb - CSIG (Additional S&H or Hazmat Fees May Apply)
NETA PART: CSIG-99940T
MFG.PART: 99940T
UNSPSC: 12352203
Manufacturer: Cell Signaling
| Size | 20 µl |
| Reactivity | M |
| Sensitivity | Endogenous |
| Molecular Weight (kDa) | 22 |
| Source/Isotype | Rabbit IgG |
| Application/Dilution | {Western Blotting: 1:1000, IHC Leica Bond: 1:50 - 1:200, Immunohistochemistry (Paraffin): 1:75 - 1:300} |
| Storage | Supplied in 10 mm sodium HEPES (pH 7.5), 150 mm NaCl, 100 µg/mL BSA, 50% glycerol and less than 0.02% sodium azide. Store at –20°C. Do not aliquot the antibody. For a carrier-free (BSA and azide free) version of this product see product #47359 |
| Specificity/Sensitivity | CD3ε (D4V8L) Rabbit mAb recognizes endogenous levels of total mouse CD3ε protein. Non-specific staining in mouse pancreas has been observed.CD3ε (D4V8L) Rabbit mAb may react weakly with human CD3ε, but is not suggested for use in immunohistochemical analysis of human tissues. Instead, CD3ε (D7A6E™) XP® Rabbit mAb #85061 is recommended for IHC analysis of human tissue samples. |
| Species Reactivity | Mouse |
| Source/Purification | Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Val31 of mouse CD3ε protein. |
| Background | When T cells encounter antigens via the T cell receptor (TCR), information about the quantity and quality of antigens is relayed to the intracellular signal transduction machinery (1). This activation process depends mainly on CD3 (Cluster of Differentiation 3), a multiunit protein complex that directly associates with the TCR. CD3 is composed of four polypeptides: ζ, γ, ε, and δ. Each of these polypeptides contains at least one immunoreceptor tyrosine-based activation motif (ITAM) (2). Engagement of the TCR complex with foreign antigens induces tyrosine phosphorylation in the ITAM motifs and phosphorylated ITAMs function as docking sites for signaling molecules such as ZAP-70 and the p85 subunit of PI-3 kinase (3,4). TCR ligation also induces a conformational change in CD3ε, such that a proline region is exposed and then associates with the adaptor protein Nck (5). |
| SKU | CSIG-99940T |
|---|---|
| Supplier Part Number | 99940T |
| UM | EA |
| UNSPSC | 12352203 |
| Manufacturer | Cell Signaling |
| MSDS URL | Click here |
| Temperature | -20C |
| CountryOfOrigin | United States |
| ProductLine | CSIG |
| Qty | 1 |
| MinOrderQty | 1 |
| Weight | 7.000000 |
| Lead Time | 5 |
| Hazardous | N |
| Energy Star | No |
| Green | No |
| Controlled | N |