Sigma-Aldrich Thrombin From Human Plasma

Biological Source

human plasma

Quality Level

100

Form

lyophilized

Specific Activity

~120 units/mg protein (At 25 °C with Chromozym TH as the substrate.)

Mol Wt

M_r~33.6 kDa

Packaging

pkg of 20 U

Manufacturer/Tradename

Roche

Optimum pH

8.2-9.0

Shipped In

wet ice

Related Categories

Albumins and Transport Proteins

Roche® Life Science Products

General Description

Thrombin is a Na⁺activated allosteric serine protease. It belongs to chymotrypsin family.Thrombin is made of two polypeptide chains of 36 (A chain) and 259 (B chain) residues that are covalently attached with a disulfide bond.Thrombin is a coagulation factor IIa. It is a serine endopeptidase that hydrolyzes peptide and ester bonds specifically at the carboxylic side of Arg.The enzyme converts fibrinogen to fibrin. The product contains EDTA and additional stabilizing agents.

Application

Thrombin has been used in the expression and purification of recombinant RELM (resistin-like moleculefamily members.[5] It has also been used in platelet spreading on fibrinogen[6][7] and immunostaining.[8]Thrombin is used in coagulation research, medical research, protein-structure analysis, and biochemical research. It has been used for the stimulation of the platelets.[8][9]

Biochem/Physiol Actions

Thrombin plays an important role in blood coagulation. It serve as a procoagulant factor while transforming fibrinogen into an insoluble fibrin clot. Thrombin can act as anticoagulant by the activation of protein C.

Physical Form

Lyophilizate

Preparation Note

Storage conditions (working solution):-15 to -25°C

Inhibitors: DFP, TLCK, PMSF, benzamidine, α₁-antitrypsin, α₂-macroglobulin, antithrombin III heparin, hirudin, and APMSF

Reconstitution

The reconstituted solution contains 20 mm EDTA.
Important note:
Use plastic vials and pipettes because thrombin will be adsorbed to glass surfaces!

Storage And Stability

Store at 2 to 8 °C. (Store dry!)

Other Notes

For life science research only. Not for use in diagnostic procedures.

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Pictograms

GHS07

Signalword

Warning

Hcodes

H332

Pcodes

P261 - P271 - P304 + P340 + P312

Hazard Classifications

Acute Tox. 4 Inhalation

Storage Class

11 - Combustible Solids

Wgk_Germany

WGK 2

Flash_Point_F

does not flash

Flash_Point_C

does not flash