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Millipore This Anti-Phospho-Pkr (Thr451) Antibody Is Validated For Use In Wb For The Detection Of Phospho-Pkr (Thr451). - Mill (Additional S&H or Hazmat Fees May Apply)
100 µL of affinity purified rabbit polyclonal IgG in Dulbecco’s PBS, containing 1.0 mg/mL BSA, 0.05% sodium azide and 50% glycerol.
Quality Level
MQ100
Applications
Application
This Anti-phospho-PKR (Thr451) Antibody is validated for use in WB for the detection of phospho-PKR (Thr451).
Key Applications
Western Blotting
Biological Information
Immunogen
Peptide corresponding to amino acid region encompassing the human phospho-PKR (Thr451)
Host
Rabbit
Specificity
Recognizes phosphorylated PKR (Thr451)
Isotype
IgG
Species Reactivity
Human
Mouse
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NM_002759.1
Gene Symbol
EIF2AK2
PRKR
PKR
MGC126524
EIF2AK1
Modifications
Phosphorylation
Purification Method
ImmunoAffinity Purified
UniProt Number
P19525
UniProt Summary
FUNCTION: SwissProt: P19525 # Following activation by double-stranded RNA in the presence of ATP, the kinase becomes autophosphorylated and can catalyze the phosphorylation of the translation initiation factor EIF2S1, which leads to an inhibition of the initiation of protein synthesis. Double-stranded RNA is generated during the course of a viral infection. SIZE: 551 amino acids; 62094 Da SUBUNIT: Homodimer. Interacts with STRBP (By similarity). Interacts with DNAJC3. Inhibited by direct interaction with viral proteins such as HCV E2, HCV NS5A and influenza A NS1. Activated by the interaction with HIV-1 Tat. PTM: Autophosphorylated on several Ser and Thr residues. Autophosphorylation of Thr-451 is dependent on Thr-446 and is stimulated by dsRNA binding and dimerization. Autophosphorylation apparently leads to the activation of the kinase. SIMILARITY: SwissProt: P19525 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. GCN2 subfamily. & Contains 2 DRBM (double-stranded RNA-binding) domains. & Contains 1 protein kinase domain.
Molecular Weight
68 kDa
Product Usage Statements
Quality Assurance
Routinely evaluated by immunoblot.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Stable for 2 years at -20°C from date of shipment. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.
