The store will not work correctly when cookies are disabled.
We use cookies to make your experience better.To comply with the new e-Privacy directive, we need to ask for your consent to set the cookies. Learn more.
NETA ALERT: Due to inclement weather our offices are closed today, Monday Feb. 23. We will not be shipping orders.
Millipore Anti-Phospho-Src (Tyr418) Antibody Detects Level Of Phospho-Src (Tyr418) & Has Been Published & Validated For Use In Ic, Ih & Wb. - Mill (Additional S&H or Hazmat Fees May Apply)
Chick embryo fibroblast (CEF) cells expressing human wild-type Src protein
Presentation
Affinity Purified rabbit polyclonal IgG in PBS (without Mg2+ and Ca2+), pH 7.3 containing 0.5 mg/mL BSA (IgG, protease free) and 0.025% sodium azide and 50% glycerol.
Quality Level
MQ100
Applications
Application
Anti-phospho-Src (Tyr418) Antibody detects level of phospho-Src (Tyr418) & has been published & validated for use in IC, IH & WB.
Key Applications
Immunocytochemistry
Immunohistochemistry
Western Blotting
Application Notes
Immunoblot Analysis: 1:1000 dilutions of this lot detected phospho-Src (Tyr418) in RIPA lysate of Src transfected CEF cells.
Biological Information
Immunogen
Peptide corresponding to amino acid region encompassing the human, mouse, rat, chicken, and frog phospho-Src (Tyr418)
Concentration
Please refer to the Certificate of Analysis for the lot-specific concentration.
Host
Rabbit
Specificity
Recognizes Src phosphorylated on Tyrosine residue 418.
Isotype
IgG
Species Reactivity
Chicken
Human
Mouse
Species Reactivity Note
Predicted to cross-react with Rat and Frog (100% homologous) based on sequence homology.
Antibody Type
Polyclonal Antibody
Entrez Gene Number
NM_005417.3
NM_198291.1
Entrez Gene Summary
This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene.
Gene Symbol
SRC
ASV
c-src
p60-Src
SRC1
c-SRC
pp60c-src
Modifications
Phosphorylation
Purification Method
ImmunoAffinity Purified
UniProt Number
P12931
UniProt Summary
SIZE: 536 amino acids; 59835 Da SUBUNIT: Interacts with DDEF1/ASAP1 via its SH3 domain. Interacts with CCPG1 (By similarity). Interacts with CDCP1, PELP1, TGFB1I1 and TOM1L2. PTM: Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. The phosphorylated tail interacts with the SH2 domain thereby repressing kinase activity. SIMILARITY: SwissProt: P12931 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. & Contains 1 protein kinase domain. & Contains 1 SH2 domain. & Contains 1 SH3 domain.
Molecular Weight
60 kDa
Product Usage Statements
Quality Assurance
Routinely evaluated by immunoblot.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Maintain for 2 years at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
