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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 07-916 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-Rap1 Antibody |
| Alternate Names |
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| Background Information | Ras-related protein Rap-1A (UniProt: P62834; also known as EC:3.6.5.2, C21KG, G-22K, GTP-binding protein smg p21A Ras-related protein Krev-1, Rap1A) is encoded by the RAP1A (also known as KREV1) gene (Gene ID: 5906) in human. The Ras superfamily is a large group of proteins that cycle between GDP- and GTP-bound forms and interact with proteins depending on the nucleotide that is bound. Two isoforms of Rap1 have been described that are known as Rap1A and Rap1B. They differ in only a few amino acids. Rap1 binds either GDP or GTP, and the change between the two states represents a molecular switch where the GTP-bound form is on and the GDP-bound form off. Rap1A, a cell membrane, lipid anchor protein, is a ubiquitously expressed protein that was first identified as a ras-tumor-suppressor gene in ras-transformed NIH 3T3 fibroblasts. It is synthesized with a short propeptide (aa 182-184) that is subsequently cleaved off to produce the mature, functional protein that has three GTP-binding sites. It is activated by guanine nucleotide-exchange factors (GEF) EPAC and EPAC2 in a cAMP-dependent manner. Rap1A counteracts the mitogenic function of Ras, at least partly because it can interact with Ras GAPs and RAF in a competitive manner. It is reported to induce morphological reversion of a cell line transformed by a Ras oncogene. (Ref.: Bos, JL., et al. (2001). Nat. Rev. Mol. Cell Biol. 2(5); 369-377) |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Purified rabbit polyclonal antibody in buffer containing 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl, and 0.1% sodium azide with 0.1% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Rap1, Cat. No. 07-916, is a Rabbit polyclonal antibody that detects Rap1a and Rap1b and is tested for use in Western Blotting. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | KLH-conjugated linear peptide corresponding to 18 amino acids from the N-terminal region of human Ras-related protein Rap-1A (RAP1A). |
| Epitope | N-terminal |
| Concentration | 1 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type. |
| Host | Rabbit |
| Specificity | This rabbit polyclonal antibody detects Ras-related protein Rap-1A (RAP1A). It targets an epitope within 18 amino acids from the N-terminal region. |
| Isotype | IgG |
| Species Reactivity |
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| Species Reactivity Note | Human, Mouse, Rat. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
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| Purification Method | ImmunoAffinity Purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: P52306 # Stimulates GDP/GTP exchange reaction of a group of small GTP-binding proteins (G proteins) including Rap1a/Rap1b, RhoA, RhoB and KRas, by stimulating the dissociation of GDP from and the subsequent binding of GTP to each small G protein. SIZE: 607 amino acids; 66403 Da SIMILARITY: SwissProt: P52306 ## Contains 5 ARM repeats. |
| Molecular Weight | ~22 kDa observed; 20.99 kDa calculated. Uncharacterized bands may be observed in some lysate(s). |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Evaluated by Western Blotting in lysate of HeLa cells treated with GDP and GTP . Western Blotting Analysis (WB): 1:1,000 dilution of this antibody detected Rap1a and Rap1b in Ral1 GDS-RBD agarose precipitated from lysate of HeLa cells treated with GDP and GTP . |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at 2-8°C from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |