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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H | IP, WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | 09-217 |
| Brand Family | Upstate |
| Trade Name |
|
| Description | Anti-Raptor Antibody |
| Alternate Names |
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| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Affinity purified rabbit polyclonal IgG in 0.02M PBS, 0.25M sodium chloride containing 0.1% sodium azide and 50% glycerol. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect Raptor using this anti-Raptor Antibody validated for use in IP & WB. |
| Key Applications |
|
| Biological Information | |
|---|---|
| Immunogen | KLH conjugated peptide encompassing the N-terminal region of human Raptor. |
| Host | Rabbit |
| Isotype | IgG |
| Species Reactivity |
|
| Species Reactivity Note | Other species have not been tested. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
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| Gene Symbol |
|
| Purification Method | ImmunoAffinity Purified |
| UniProt Number |
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| UniProt Summary | FUNCTION: SwissProt: Q8N122 # Participates in the FRAP1 pathway and associates in a near stoichiometric ratio with FRAP1 to form a nutrient-sensitive complex (NSC). Plays a pivotal role as a scaffold protein in the FRAP1-signaling pathway and this interaction is essential for the catalyzed phosphorylation of EIF4EBP1. Has a positive role in nutrient-stimulated signaling to the downstream effector RPS6KB1. Under nutrient-deprived conditions, serves as a negative regulator of FRAP1 kinase activity. Regulation of the interaction with FRAP1 is a critical mechanism by which cells coordinate the rate of cell growth and maintenance of cell size with different environmental conditions. SIZE: 1335 amino acids; 149038 Da SUBUNIT: Binds directly 4EBP1 and RPS6KB1 independently of its association with FRAP1. Binds preferentially to poorly or non- phosphorylated form of EIF4EBP1, and this binding is critical to the ability of FRAP1 to catalyze phosphorylation. Complex with FRAP1 physically interacts under both leucine-rich and -poor conditions and therefore in at least two nutrient-determined states with different stability. TISSUE SPECIFICITY: Highly expressed in skeletal muscle, and in a lesser extent in brain, lung, small intestine, kidney and placenta. SIMILARITY: SwissProt: Q8N122 ## Contains 7 WD repeats. MISCELLANEOUS: Rapamycin destabilizes the interaction with FRAP1 regardless of nutrient availability, and its potency for dissociation is increased under nutrient-rich conditions. This action uncouples FRAP1 from its substrates, and inhibits FRAP1 signaling without altering its intrinsic catalytic activity. |
| Molecular Weight | 150 kDa |
| Product Usage Statements | |
|---|---|
| Quality Assurance | Routinely evaluated by immunoblot. |
| Usage Statement |
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| Storage and Shipping Information | |
|---|---|
| Storage Conditions | 2 years at -20°C from date of shipment |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |