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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M | ELISA, RIA, IHC | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB2034 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Laminin Antibody |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | The purified antibody is supplied in a buffer containing a mixture of 0.1M Citrate, 0.1M potassium phosphate, at a pH of 7.2-7.4 and 10µg/ml of antibiotics and antimytotics |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Laminin Antibody detects level of Laminin & has been published & validated for use in ELISA, RIA, IH. |
| Key Applications |
|
| Application Notes | IFA (with fluorescein anti-rabbit IgG conjugate) - use diluted at ≥ 1:80 on frozen mouse tissues (skin, liver, etc.). IHC (paraffin) - use diluted at ≥ 1:500 on fixed, paraffin-embedded mouse tissues (skin, liver, etc). (immunoperoxidase technique with the Envision / Dako kit); ELISA - use diluted at ≥ 1:200 (OD ≥ 500). Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Laminin extracted and purified from EHC mouse tumor tissues. |
| Concentration | Please refer to the Certificate of Analysis for the lot-specific concentration. |
| Host | Rabbit |
| Specificity | Laminin extracted and purified from mouse tumor tissues. Antibody shows less than 0.1% reactivity with mouse collagen Types I, II, and IV; rat collagen Types I, III, and V; rat and human elastin; mouse fibronectin and laminin by radioimmunoassay. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Gene Symbol |
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| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P25391 # Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components. SIZE: 3075 amino acids; 337158 Da SUBUNIT: Laminin is a complex glycoprotein, consisting of three different polypeptide chains (alpha, beta, gamma), which are bound to each other by disulfide bonds into a cross-shaped molecule comprising one long and three short arms with globules at each end. Alpha-1 is a subunit of laminin-1 (EHS laminin) and laminin-3 (S-laminin). SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix, basement membrane. Note=Major component. DOMAIN: SwissProt: P25391 The alpha-helical domains I and II are thought to interact with other laminin chains to form a coiled coil structure. & Domains VI, IV and G are globular. SIMILARITY: Contains 17 laminin EGF-like domains. & Contains 5 laminin G-like domains. & Contains 2 laminin IV type A domains. & Contains 1 laminin N-terminal domain. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain frozen at -20°C for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |