Special Offers
100% Performance Guaranteed
Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | IP, WB | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB5699 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Cysteine-rich Motor Neuron 1 Antibody |
| Alternate Names |
|
| Product Information | |
|---|---|
| Format | Serum |
| Presentation | Rabbit serum. Liquid. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Cysteine-rich Motor Neuron 1 Antibody detects level of Cysteine-rich Motor Neuron 1 & has been published & validated for use in IP & WB. |
| Key Applications |
|
| Application Notes | Western blot: 1:1,000 using ECL. The antibody reacts with proteins of ~120 kDa. Preferred gel percentage is 7.5%. Suggested blocking buffer is PBS containing 5% non-fat milk. Suggested dilution buffer is PBS containing 1% non-fat milk. Immunoprecipitation: 5 μL to 1 mL of cell extract. Preferred tissue/cell lysis buffer is RIPA. Suggested incubation time is 2-4 hours. Suggested capture agent is Protein A. Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Recombinant protein from the cytoplasmic tail of mouse Crim1. |
| Host | Rabbit |
| Specificity | Crim1 SUBCELLULAR LOCALIZATION: Intracellular and Membrane associated. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | Motor neurons are among the earliest neurons to appear after the commencement of cell patterning and the beginning of cell differentiation. Differentiation occurs in a ventral-to-dorsal gradient and is mediated, at least in part, by the concentration of ventrally expressed sonic hedgehog protein (SHH; MIM 600725). Dorsally expressed factors, such as members of the bone morphogenic protein (e.g., BMP4; MIM 112262) and transforming growth factor-beta (e.g., TGFB1; MIM 190180) families, can repress the induction of these neurons. CRIM1 may interact with growth factors implicated in motor neuron differentiation and survival (Kolle et al., 2000 [PubMed 10642437]).[supplied by OMIM] |
| Gene Symbol |
|
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: Q9NZV1 # May play a role in CNS development by interacting with growth factors implicated in motor neuron differentiation and survival. May play a role in capillary formation and maintenance during angiogenesis. Modulates BMP activity by affecting its processing and delivery to the cell surface. SIZE: 1036 amino acids; 113738 Da SUBUNIT: Interacts with BMP4 and BMP7. SUBCELLULAR LOCATION: Processed cysteine-rich motor neuron 1 protein: Secreted. & Cell membrane; Single-pass type I membrane protein. TISSUE SPECIFICITY: Expressed in pancreas, kidney, skeletal muscle, lung, placenta, brain, heart, spleen, liver and small intestine. Expressed in blood vessels (at protein level). PTM: N-glycosylated. SIMILARITY: SwissProt: Q9NZV1 ## Contains 4 antistasin-like domains. & Contains 1 IGFBP N-terminal domain. & Contains 6 VWFC domains. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -20°C in undiluted aliquots for up to 12 months after date of receipt. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 100 µL |