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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | IHC, WB | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB5702 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-HES-1 Antibody |
| Alternate Names |
|
| Background Information | Hes1 is a transcriptional repressor of genes that require a bHLH protein for their transcription. It may act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1. Hes1 is expressed in developing neuroectodermal and endodermal endocrine tissues, and Hes1 deficient embryos show severe defects in neuronal development, as well as pancreatic hypoplasia. |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Control |
|
| Presentation | Purified rabbit polyclonal in buffer containing a solution of 50% saturated ammonium sulfate and PBS containing no preservatives. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | This Anti-HES-1 Antibody is validated for use in IH, WB for the detection of HES-1. |
| Key Applications |
|
| Application Notes | Western Blot: 1:200-1:1,000 dilution using ECL. Immunohistochemistry: 1:200-1:1,000 dilution of a previous lot worked. Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Synthetic peptide. |
| Host | Rabbit |
| Specificity | Recognizes HES-1 (Hairy family), a Helix-loop-helix class of transcription factor. |
| Species Reactivity |
|
| Species Reactivity Note | Human and rodent. Reactivity with other species has not been confirmed. |
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This protein belongs to the basic helix-loop-helix family of transcription factors. It is a transcriptional repressor of genes that require a bHLH protein for their transcription. The protein has a particular type of basic domain that contains a helix interrupting protein that binds to the N-box rather than the canonical E-box. |
| Gene Symbol |
|
| Purification Method | ImmunoAffinity Purified |
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: Q14469 # Transcriptional repressor of genes that require a bHLH protein for their transcription. May act as a negative regulator of myogenesis by inhibiting the functions of MYOD1 and ASH1 (By similarity). SIZE: 280 amino acids; 29541 Da SUBUNIT: Transcription repression requires formation of a complex with a corepressor protein (Groucho) such as TLE2 (By similarity). Interacts with SIRT1. SUBCELLULAR LOCATION: Nucleus. DOMAIN: SwissProt: Q14469 Has a particular type of basic domain (presence of a helix-interrupting proline) that binds to the N-box (CACNAG), rather than the canonical E-box (CANNTG). & The C-terminal WRPW motif is a transcriptional repression domain necessary for the interaction with Groucho, a transcriptional corepressor recruited to specific target DNA by Hairy-related proteins. SIMILARITY: Contains 1 basic helix-loop-helix (bHLH) domain. & Contains 1 Orange domain. ... hide » see more » |
| Molecular Weight | 29 kDa |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Stable for 1 year at -80ºC from date of receipt. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |