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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M | WB, IHC | Rb | Affinity Purified | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB5714 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-Hey 1/HRT1 Antibody |
| Product Information | |
|---|---|
| Format | Affinity Purified |
| Presentation | Precipitated antibody in a solution of 50% saturated ammonium sulfate and PBS containing no preservatives. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-Hey 1/HRT1 Antibody detects level of Hey 1/HRT1 & has been published & validated for use in WB, IH. |
| Key Applications |
|
| Application Notes | Western blot: 1:200-1:1,000 using ECL. Immunohistochemistry: 1:200-1:1,000. Optimal working dilutions must be determined by the end user. |
| Biological Information | |
|---|---|
| Immunogen | Synthetic peptide, amino acids 207-220 of human Hey 1 / HRT1. |
| Host | Rabbit |
| Specificity | Recognizes Hey 1 / HRT1, a Helix-loop-helix class of transcription factor. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | This gene encodes a nuclear protein belonging to the hairy and enhancer of split-related (HESR) family of basic helix-loop-helix (bHLH)-type transcriptional repressors. Expression of this gene is induced by the Notch and c-Jun signal transduction pathways. Two similar and redundant genes in mouse are required for embryonic cardiovascular development, and are also implicated in neurogenesis and somitogenesis. Alternative splicing results in multiple transcript variants. |
| Gene Symbol |
|
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: Q9Y5J3 # Downstream effector of Notch signaling which may be required for cardiovascular development. Transcriptional repressor which binds preferentially to the canonical E box sequence 5- CACGTG-3. Represses transcription by the cardiac transcriptional activators GATA4 and GATA6. SIZE: 304 amino acids; 32613 Da SUBUNIT: Self-associates (By similarity). Interacts with HES1 and HEYL (By similarity). Interacts with HDAC1, NCOR1 and SIN3A (By similarity). Interacts with GATA4 and GATA6 (By similarity). SUBCELLULAR LOCATION: Nucleus (By similarity). TISSUE SPECIFICITY: Expressed in the somitic mesoderm, the central nervous system, the kidney, the heart, nasal epithelium, and limbs. SIMILARITY: SwissProt: Q9Y5J3 ## Belongs to the HEY family. & Contains 1 basic helix-loop-helix (bHLH) domain. & Contains 1 Orange domain. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain unopened vial at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles. The rehydrated antibody solutions can be stored undiluted at 2-8°C for 2 months without any significant loss of activity. Note, the solution is not sterile, thus care should be taken if product is stored at 2-8°C. For storage at -20°C, the addition of an equal volume of glycerol can be used, however, it is recommended that ACS grade or higher glycerol be used, as significant loss of activity can occur if the glycerol used is not of high quality. For freezing, it is recommended that the rehydrated antibody solution be further diluted 1:1 with a 2% BSA (fraction V, highest-grade available) solution made with the rehydration buffer. The resulting 1% BSA/antibody solution can be aliquoted and stored frozen at -70°C for up to 6 months. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 100 µg |