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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R | ICC, IHC, WB | Gp | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB5894 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-P2X2 Receptor Antibody |
| Product Information | |
|---|---|
| Format | Serum |
| Presentation | Serum. Liquid. Contains 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Anti-P2X2 Receptor Antibody is an antibody against P2X2 Receptor for use in IC, IH & WB. |
| Key Applications |
|
| Application Notes | Immunoblotting: 1:500 Immunohistochemistry: 1:500. Immunocytochemistry: 1:500. Optimal working dilutions must be determined by end user. APPLICATION NOTES FOR AB5894 IMMUNOHISTOCHEMISTRY Male Sprague-Dawley rats (b.wt. 100-150g) were anesthetized with sodium pentobarbital and perfused via the ascending aorta with: 1) 50 mL of Ca2+-free Tyrode+s solution followed by 2) a formalin-picric acid fixative (4% paraformaldehyde with 0.4% picric acid in 0.16 M phosphate buffer, pH 6.9) and 3) 10% sucrose in PBS as a cryo-protectant. Tissues were rapidly dissected out and stored overnight in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose. Slide-mounted tissue sections were incubated with blocking buffer for 1 hour at room temperature. Primary antibody was diluted in blocking buffer to the appropriate working dilution. Blocking buffer was removed and the slides were then incubated at 2-8°C for 18-24 hours with AB5894 (1:500). After rinsing in PBS 3 times sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope. IMMUNOCYTOCHEMISTRYP 2X2 transfected cells were processed for indirect immunofluorescence. Media was removed and cells were gently washed 3 times with serum-free media. Following fixation procedure, cells were processed for indirect immunofluorescence as above WESTERN BLOTTING Cell membrane extracts were examined by electrophoresis (8% acrylamide) with SDS under reducing conditions and transferred to a nylon membrane. Membranes were blocked for 1 hour at 2-8°C with 0.1% Tween 20 and 2.5% milk powder (w/v) in PBS. Membranes were incubated with AB5894 diluted 1:500 with same buffer overnight at 2-8°C. Membranes were rinsed and incubated with HRP conjugated secondary antibody for 1 hour at room temperature. Following rinsing the membranes were processed using enhanced chemiluminescence. |
| Biological Information | |
|---|---|
| Immunogen | A 13 amino acid peptide corresponding to amino acids 460-472 from the carboxy-terminus of the rat P2X2 Receptor protein. Control peptide: Catalog Number AG354 |
| Host | Guinea Pig |
| Specificity | P2X2 Receptor. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel. Binding to ATP mediates synaptic transmission between neurons and from neurons to smooth muscle. Six transcript variants encoding six distinct isoforms have been identified for this gene. |
| Gene Symbol |
|
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: Q9UBL9 # Binding of this ligand gated ion channel to ATP mediates synaptic transmission between neurons and from neurons to smooth muscle. SIZE: 471 amino acids; 51754 Da SUBUNIT: Homo- or heteropolymers. SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. SIMILARITY: SwissProt: Q9UBL9 ## Belongs to the P2X receptor family. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles. |
| Packaging Information | |
|---|---|
| Material Size | 50 µL |