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Key Specifications Table
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| R, Pm, H | ICC, IHC, WB | Rb | Serum | Polyclonal Antibody |
| Description | |
|---|---|
| Catalogue Number | AB5895 |
| Brand Family | Chemicon® |
| Trade Name |
|
| Description | Anti-P2X3 Receptor Antibody, pain |
| Product Information | |
|---|---|
| Format | Serum |
| Presentation | Rabbit serum. Liquid. Contains 0.05% sodium azide. |
| Quality Level | MQ100 |
| Applications | |
|---|---|
| Application | Detect P2X3 Receptor using this Anti-P2X3 Receptor Antibody, pain validated for use in IC, IH & WB. |
| Key Applications |
|
| Application Notes | Immunoblotting: 1:500-1:1,000 Immunohistochemistry: 1:500-1:1,000 Immunocytochemistry: 1:500-1:1,000 Optimal working dilutions must be determined by the end user. APPLICATION NOTES FOR AB5895 IMMUNOHISTOCHEMISTRY Male Sprague-Dawley rats (b.wt. 100-150g) were anesthetized with sodium pentobarbital and perfused via the ascending aorta with: 1) 50 mL of Ca2+-free Tyrode+s solution followed by 2) fixative, and 3) 10% sucrose in PBS as a cryo-protectant. Tissues were rapidly dissected out and stored overnight in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose. Slide-mounted tissue sections were incubated with blocking buffer for 1 hour at room temperature. Primary antibody was diluted in blocking buffer to the appropriate working dilution. Blocking buffer was removed and the slides were then incubated at 2-8°C for 18-24 hours with AB5895. After rinsing in PBS 3 times sections were incubated for 60 minutes at room temperature with Cy3-conjugated secondary antibodies. After mounting in a mixture of PBS and glycerol (1:3) containing 0.1% p-phenylenediamine, sections were examined with a Nikon Microphot-SA epifluorescence microscope. IMMUNOCYTOCHEMISTRY P2X3 transfected cells were processed for indirect immunofluorescence. Media was removed and cells were gently washed 3 times with serum-free media. Following fixation procedure, cells were processed for indirect immunofluorescence as described above. |
| Biological Information | |
|---|---|
| Immunogen | Synthetic peptide corresponding to the C-terminus of rat P2X3. |
| Host | Rabbit |
| Specificity | P2X3 SPECIES REACTIVITIES: Rat, mouse and primate. Other species have not been tested. |
| Species Reactivity |
|
| Antibody Type | Polyclonal Antibody |
| Entrez Gene Number |
|
| Entrez Gene Summary | The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel and may transduce ATP-evoked nociceptor activation. Mouse studies suggest that this receptor is important for peripheral pain responses, and also participates in pathways controlling urinary bladder volume reflexes. It is possible that the development of selective antagonists for this receptor may have a therapeutic potential in pain relief and in the treatment of disorders of urine storage. |
| Gene Symbol |
|
| UniProt Number |
|
| UniProt Summary | FUNCTION: SwissProt: P56373 # Receptor for ATP that acts as a ligand gated ion channel. SIZE: 397 amino acids; 44230 Da SUBUNIT: Homo- or heteropolymers (By similarity). SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein. SIMILARITY: SwissProt: P56373 ## Belongs to the P2X receptor family. |
| Product Usage Statements | |
|---|---|
| Usage Statement |
|
| Storage and Shipping Information | |
|---|---|
| Storage Conditions | Maintain at -20°C in undiluted for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self-defrosting freezer. |
| Packaging Information | |
|---|---|
| Material Size | 50 µL |